If you are interested in developing new super-resolution microscopy methods, and especially have skills in imaging software and instrument development, we would like to hear from you. For queries, please get in touch directly with PI: Dr Izzy Jayasinghe via i.jayasinghe[at]sheffield.ac.uk.
Our latest pre-print is now online, where we evaluated two peptide ligands of the cardiac ryanodine receptor (RyR) as super-resolution imaging probes. The research was conducted in collaboration with one of our industrial partners, Badrilla Ltd., during the industrial internship of iCASE PhD student Tom Sheard funded through the DiMeN DTP.
The peptides used were the scorpion venom toxin imperacalcin, and CPVT-mimicking DPc10. We evaluated the specificity of the fluorescent peptide conjugates for the target RyR2 in fluorescent imaging experiments, in terms of colocalisation with RyR2 immunolabelling, with confocal and expansion microscopy.
We also performed structural work looking at the DPc10 binding site on RyR2 atomic models to learn more about the peptide’s mechanism of disturbance (DPc10 binding to RyR2 causes domain unzipping and destabilises the channel).
Thanks to the host company Badrilla, the DiMeN DTP for funding Tom’s industrial placement, and the other authors for help with the research.
Our new book chapter has been published, detailing the enhanced expansion microscopy technique (EExM). The protocol offers a route to visualising cellular ultrastructures at a resolution of 15 nm in-plane (and ~ 35 nm axially) with a hydrogel-based molecular-scale imprint of the fluorescence pattern.
We detail how we used EExM to explore structural and biochemical remodelling in cardiomyocytes. The chapter includes a step-by-step protocol, reagent list and troubleshooting tips.
We have launched a brief survey examining the needs in the Life Sciences around the use or potential utility of optical (fluorescence) microscopies. This takes ~ 6 minutes to complete and gives you an opportunity to stay in touch or engaged directly with the new developments of the newly launched UKRI Future Leader Fellowship within the Applied Biophotonics Group. Whether you are an experienced microscopist or never had the opportunity to use optical imaging, can such microscopies add value to your research or day-to-day work? If so, what holds you back? If you work within any area of Life Sciences, we would like to hear about your experiences. Please take the survey and let us know.
1st of May – We have officially moved our research operations to the Department of Molecular Biology & Biotechnology in our new home institute, the University of Sheffield. We will be based in the famous Firth Court, working closely with a wide range of interdisciplinary research groups and the IMAGINE imaging consortium.
The UK is currently in the midst of the lockdown due to the ongoing COVID-19 pandemic. Normal operations will therefore begin in the coming months. There will be a number of professional and study opportunities within our team coming very soon. So watch this space!