New book chapter detailing EExM in Methods in Cell Biology

How we prepared EExM samples

Our new book chapter has been published, detailing the enhanced expansion microscopy technique (EExM). The protocol offers a route to visualising cellular ultrastructures at a resolution of 15 nm in-plane (and ~ 35 nm axially) with a hydrogel-based molecular-scale imprint of the fluorescence pattern.

We detail how we used EExM to explore structural and biochemical remodelling in cardiomyocytes. The chapter includes a step-by-step protocol, reagent list and troubleshooting tips.

Access the paper published in Methods in Cell Biology at:

Any difficulties accessing the article please contact the Tom Sheard via email at: bs11ts[at]

This is the first book chapter for PhD student Tom Sheard (@tmdsheard on Twitter).


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