Our new book chapter has been published, detailing the enhanced expansion microscopy technique (EExM). The protocol offers a route to visualising cellular ultrastructures at a resolution of 15 nm in-plane (and ~ 35 nm axially) with a hydrogel-based molecular-scale imprint of the fluorescence pattern.
We detail how we used EExM to explore structural and biochemical remodelling in cardiomyocytes. The chapter includes a step-by-step protocol, reagent list and troubleshooting tips.
Access the paper published in Methods in Cell Biology at: https://sciencedirect.com/science/article/pii/S0091679X20301084.
Any difficulties accessing the article please contact the Tom Sheard via email at: bs11ts[at]leeds.ac.uk
This is the first book chapter for PhD student Tom Sheard (@tmdsheard on Twitter).